255 PRONUCLEUS FORMATION IN RAT ZONA-FREE OOCYTES CO-CULTURED WITH HOMOLOGOUS POST-THAW SPERMATOZOA
نویسندگان
چکیده
منابع مشابه
Ultrastructural study of the interactions and fusion of ram spermatozoa with zona-free hamster oocytes.
The interaction of preincubated ram sperm with zona-free hamster oocytes was studied by scanning and transmission electron microscopy. The behaviour of the sperm cells was quite different, depending on whether the acrosomal reaction had taken place or not. The apical ridge of intact spermatozoa contacted the oocyte surface, and egg microvilli spread onto the anterior segment but no fusion ensue...
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Cryopreservation of boar semen is still considered suboptimal due to lower fertility as compared with fresh samples when glycerol, a permeating cryoprotectant, is used. Trehalose is a non-permeable cryoprotectant and nonreducing disaccharide known to stabilize proteins and biologic membranes. The aim of this study was to evaluate the cryosurvival and in vitro penetrability of boar spermatozoa w...
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BACKGROUND Successful cryopreservation of rat spermatozoa from various strains still remains a challenge. The objective of this study was to determine if combinations of OptiPrep™ (iodixanol) and adenosine 5'-triphosphate (ATP) can improve rat sperm function during the cryopreservation procedure. METHODS Epididymal rat spermatozoa were frozen under different OptiPrep™ concentrations (0, 1, 2,...
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Multipotent mesenchymal stromal cells (MSC) are receiving increased attention for their non-progenitor immunomodulatory potential. Cryopreservation is commonly used for long-term storage of MSC. Post-thaw MSC proliferation is associated with a lag-phase in vitro. How this lag-phase affect MSC immunomodulatory properties is unknown. We hypothesized that in vitro there is no difference in lymphoc...
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ژورنال
عنوان ژورنال: Reproduction, Fertility and Development
سال: 2005
ISSN: 1031-3613
DOI: 10.1071/rdv17n2ab255